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DDX3Y Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DDX3Y Antibody (N-term) AP12787a
    DDX3Y Antibody (N-term) (Cat. #AP12787a) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the DDX3Y antibody detected the DDX3Y protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15523
Other Accession NP_001116137.1, NP_004651.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 73154 Da
Antigen Region 90-119 aa
Additional Information
Gene ID 8653
Other Names ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomal, DDX3Y, DBY
Target/Specificity This DDX3Y antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the N-terminal region of human DDX3Y.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX3Y Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX3Y
Synonyms DBY
Function Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner
Tissue Location Testis-specific. Expressed predominantly in spermatogonia.
Research Areas
Citations (0)

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DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified.


Rosinski, K.V., et al. Blood 111(9):4817-4826(2008)
Lardone, M.C., et al. Mol. Hum. Reprod. 13(10):705-712(2007)
Pope, S.N., et al. J. Steroid Biochem. Mol. Biol. 94 (1-3), 203-208 (2005) :
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Ficarro, S.B., et al. Rapid Commun. Mass Spectrom. 19(1):57-71(2005)

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Cat# AP12787a
(40 western blots)
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