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DULLARD Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DULLARD Antibody (Center) AP12805c
    DULLARD Antibody (Center) (Cat. #AP12805c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DULLARD antibody detected the DULLARD protein (arrow).
    detail
  • IHC-P - DULLARD Antibody (Center) AP12805c
    DULLARD Antibdy (Center) (Cat. #AP12805c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DULLARD Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - DULLARD Antibody (Center) AP12805c
    DULLARD Antibody (Center) (Cat. #AP12805c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O95476
Other Accession Q3B7T6, Q3TP92, Q1RMV9, NP_001137247.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 28377 Da
Antigen Region 131-160 aa
Additional Information
Gene ID 23399
Other Names CTD nuclear envelope phosphatase 1, Serine/threonine-protein phosphatase dullard, CTDNEP1, DULLARD
Target/Specificity This DULLARD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the Central region of human DULLARD.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDULLARD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTDNEP1
Synonyms DULLARD
Function Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein
Tissue Location Muscle specific with lower expression in other metabolic tissues.
Research Areas
Citations (0)
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Background

Serine/threonine phosphatase which may be required for proper nuclear membrane morphology. Involved in LPIN1 dephosphorylation. May antagonize BMP signaling.

References

Kim, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 104(16):6596-6601(2007)
Zhang, Y., et al. Mol. Cell 24(5):759-770(2006)
Satow, R., et al. Dev. Cell 11(6):763-774(2006)
Satow, R., et al. Biochem. Biophys. Res. Commun. 295(1):85-91(2002)

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$ 365.00
$ 140.00
Cat# AP12805c
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