RFX2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P48378 |
Other Accession | NP_000626.2, NP_602309.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 79987 Da |
Antigen Region | 669-698 aa |
Gene ID | 5990 |
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Other Names | DNA-binding protein RFX2, Regulatory factor X 2, RFX2 |
Target/Specificity | This RFX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-698 amino acids from the C-terminal region of human RFX2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RFX2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RFX2 |
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Function | Transcription factor that acts as a key regulator of spermatogenesis. Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (By similarity). Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters (PubMed:10330134). Probably activates transcription of the testis-specific histone gene H1-6 (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:B2GV50, ECO:0000255|PROSITE-ProRule:PRU00858}. Cytoplasm {ECO:0000250|UniProtKB:B2GV50}. Note=Mainly expressed in the nucleus and at lower level in cytoplasm. {ECO:0000250|UniProtKB:B2GV50} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites.
References
Purvis, T.L., et al. Gene 460 (1-2), 20-29 (2010) :
Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Horvath, G.C., et al. Biol. Reprod. 71(5):1551-1559(2004)
Maijgren, S., et al. Arch. Dermatol. Res. 295(11):482-489(2004)
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