|Application ||WB, E|
|Other Accession||NP_758516.1, NP_001034681.1|
|Calculated MW||44819 Da|
|Antigen Region||383-411 aa|
|Other Names||Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, SMP-1, Sperm membrane protein BS-84, SPAG8|
|Target/Specificity||This SPAG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-411 amino acids from the C-terminal region of human SPAG8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPAG8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in fertility and microtubule formation through interaction with RANBP9.|
|Cellular Location||Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Acrosomal region of the head of mature sperm cells|
|Tissue Location||Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine|
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Provided below are standard protocols that you may find useful for product applications.
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
Wu, H., et al. FEBS Lett. 584(13):2807-2815(2010)
Li, R., et al. Cell Biochem. Funct. 27(5):264-268(2009)
Cheng, G.Y., et al. Asian J. Androl. 9(1):23-29(2007)
Tang, X., et al. J. Mol. Med. 82(6):383-388(2004)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
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