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CHST8 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CHST8 Antibody (Center) AP12842c
    CHST8 Antibody (Center) (Cat. #AP12842c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST8 antibody detected the CHST8 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H2A9
Other Accession NP_071912.2, NP_001121367.1, NP_001121368.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48834 Da
Antigen Region 219-247 aa
Additional Information
Gene ID 64377
Other Names Carbohydrate sulfotransferase 8, 282-, GalNAc-4-O-sulfotransferase 1, GalNAc-4-ST1, GalNAc4ST-1, N-acetylgalactosamine-4-O-sulfotransferase 1, CHST8
Target/Specificity This CHST8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-247 amino acids from the Central region of human CHST8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHST8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST8
Function Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Predominantly expressed in pituitary gland. In brain, it is expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus and spinal cord. Expressed in the epidermis. Expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary gland and trachea
Research Areas
Citations (0)

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Sulfate groups in carbohydrates confer highly specific functions on glycoproteins, glycolipids, and proteoglycans and are critical for cell-cell interaction, signal transduction, and embryonic development. Sulfotransferases, such as CHST8, carry out sulfation of carbohydrates (Hiraoka et al., 2001 [PubMed 11445554]).


Melen, E., et al. J. Allergy Clin. Immunol. 126(3):631-637(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Thorleifsson, G., et al. Nat. Genet. 41(1):18-24(2009)
Boregowda, R.K., et al. Glycobiology 15(12):1349-1358(2005)
Barret, A., et al. J. Biol. Chem. 280(11):10516-10523(2005)

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$ 295.00
$ 99.00
Cat# AP12842c
(40 western blots)
Availability: In Stock
Bulk Size
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