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GUF1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GUF1 Antibody (N-term) AP12858A
    Anti-GUF1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GUF1 Antibody (N-term) AP12858A
    GUF1 Antibody (N-term) (Cat. #AP12858a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GUF1 antibody detected the GUF1 protein (arrow).
  • IHC-P - GUF1 Antibody (N-term) AP12858A
    GUF1 Antibody (N-term) (Cat. #AP12858a)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GUF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8N442
Other Accession NP_068746.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 74328 Da
Antigen Region 183-212 aa
Additional Information
Gene ID 60558
Other Names Translation factor GUF1, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03137}, 365-, Elongation factor 4 homolog {ECO:0000255|HAMAP-Rule:MF_03137}, EF-4 {ECO:0000255|HAMAP-Rule:MF_03137}, GTPase GUF1 {ECO:0000255|HAMAP-Rule:MF_03137}, Ribosomal back-translocase {ECO:0000255|HAMAP-Rule:MF_03137}, GUF1 {ECO:0000255|HAMAP-Rule:MF_03137}
Target/Specificity This GUF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the N-terminal region of human GUF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGUF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GUF1 {ECO:0000255|HAMAP-Rule:MF_03137}
Function Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
Cellular Location Mitochondrion inner membrane {ECO:0000255|HAMAP-Rule:MF_03137}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03137}; Matrix side {ECO:0000255|HAMAP- Rule:MF_03137}
Citations (0)

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Vasilescu, J., et al. J. Proteome Res. 6(1):298-305(2007)
Kiser, G.L., et al. Yeast 11(13):1311-1316(1995)

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$ 295.00
$ 99.00
Cat# AP12858A
(40 western blots)
Availability: In Stock
Bulk Size
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