GUF1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q8N442 |
Other Accession | NP_068746.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 74328 Da |
Antigen Region | 183-212 aa |
Gene ID | 60558 |
---|---|
Other Names | Translation factor GUF1, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03137}, 365-, Elongation factor 4 homolog {ECO:0000255|HAMAP-Rule:MF_03137}, EF-4 {ECO:0000255|HAMAP-Rule:MF_03137}, GTPase GUF1 {ECO:0000255|HAMAP-Rule:MF_03137}, Ribosomal back-translocase {ECO:0000255|HAMAP-Rule:MF_03137}, GUF1 {ECO:0000255|HAMAP-Rule:MF_03137} |
Target/Specificity | This GUF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the N-terminal region of human GUF1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GUF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GUF1 {ECO:0000255|HAMAP-Rule:MF_03137} |
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Function | Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner. |
Cellular Location | Mitochondrion inner membrane {ECO:0000255|HAMAP- Rule:MF_03137}; Peripheral membrane protein {ECO:0000255|HAMAP- Rule:MF_03137}; Matrix side {ECO:0000255|HAMAP-Rule:MF_03137} |
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Provided below are standard protocols that you may find useful for product applications.
References
Vasilescu, J., et al. J. Proteome Res. 6(1):298-305(2007)
Kiser, G.L., et al. Yeast 11(13):1311-1316(1995)
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