|Application ||WB, E|
|Calculated MW||10820 Da|
|Antigen Region||1-30 aa|
|Other Names||Small ubiquitin-related modifier 2-A, SUMO-2-A, sumo2-a, smt3h2|
|Target/Specificity||This Xenopus SUMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Xenopus SUMO2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Xenopus SUMO2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex sae1-sae2 and linkage to the E2 enzyme ube2i, and can be promoted by an E3 ligase such as pias1-4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric sumo2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
SUMO2 is a ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. SUMO2 does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. This protein plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS isoforms 1-4.
Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10 Review.
Hochstrasser M. Cell. 2001 107(1):5-8. Review.
Kahyo T, et al., Mol Cell. 2001 Sep;8(3):713-8.
Yeh ET, et al., Gene. 2000 May 2;248(1-2):1-14. Review.
Keane,M.M., et al., Oncogene 18 (22), 3365-3375 (1999)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.