|Application ||WB, E|
|Calculated MW||61002 Da|
|Antigen Region||507-536 aa|
|Other Names||Nectin-3, CDw113, Poliovirus receptor-related protein 3, CD113, PVRL3, PRR3|
|Target/Specificity||This PVRL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 507-536 amino acids from the C-terminal region of human PVRL3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PVRL3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis- mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.|
|Cellular Location||Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane|
|Tissue Location||Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Yu, X., et al. Nat. Immunol. 10(1):48-57(2009)
Fujito, T., et al. J. Cell Biol. 171(1):165-173(2005)
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