|Application ||WB, E|
|Calculated MW||61002 Da|
|Antigen Region||507-536 aa|
|Other Names||Nectin-3, CDw113, Poliovirus receptor-related protein 3, CD113, PVRL3, PRR3|
|Target/Specificity||This PVRL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 507-536 amino acids from the C-terminal region of human PVRL3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PVRL3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis- mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.|
|Cellular Location||Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane|
|Tissue Location||Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Yu, X., et al. Nat. Immunol. 10(1):48-57(2009)
Fujito, T., et al. J. Cell Biol. 171(1):165-173(2005)
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