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SP140 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P, WB, E |
|---|---|
| Primary Accession | Q13342 |
| Other Accession | NP_009168.4 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 98223 Da |
| Antigen Region | 272-301 aa |
| Gene ID | 11262 |
|---|---|
| Other Names | Nuclear body protein SP140 {ECO:0000312|HGNC:HGNC:17133}, Lymphoid-restricted homolog of Sp100, LYSp100, Nuclear autoantigen Sp-140, Speckled 140 kDa, SP140 (HGNC:17133) |
| Target/Specificity | This SP140 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the Central region of human SP140. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SP140 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SP140 (HGNC:17133) |
|---|---|
| Function | Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body (PubMed:8910577). May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection (PubMed:8910577). May play a role in chromatin-mediated regulation of gene expression although it does not bind to histone H3 tails (PubMed:24267382). |
| Cellular Location | Nucleus. Nucleus, PML body Cytoplasm. Note=Localized to nuclear structures termed LANDS, for LYSp100-associated nuclear domains. LANDS are globular, electron-dense structures most often found in the nucleoplasm, but also found at the nuclear membrane and in the cytoplasm, suggesting that these structures may traffic between the cytoplasm and the nucleus (PubMed:8695863). Also colocalizes with PML in a subset of PML nuclear bodies (PubMed:8910577) |
| Tissue Location | High levels in spleen and peripheral blood leukocytes, much lower levels in tonsils, thymus, prostate, ovary, small intestine, and colon (PubMed:8695863, PubMed:8910577). Very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas (PubMed:8910577). Not detected in brain, liver and muscle (PubMed:8695863). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection.
References
Slager, S.L., et al. Cancer Epidemiol. Biomarkers Prev. 19(4):1098-1102(2010)
Di Bernardo, M.C., et al. Nat. Genet. 40(10):1204-1210(2008)
Madani, N., et al. J. Virol. 76(21):11133-11138(2002)
Bloch, D.B., et al. J. Biol. Chem. 271(46):29198-29204(1996)
Dent, A.L., et al. Blood 88(4):1423-1426(1996)
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