|Application ||WB, E|
|Other Accession||Q0P5L8, NP_055106.1|
|Calculated MW||45285 Da|
|Antigen Region||101-128 aa|
|Other Names||2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL|
|Target/Specificity||This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-128 amino acids from the N-terminal region of human GCAT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GCAT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion. Nucleus. Note=Translocates to the nucleus upon cold and osmotic stress|
|Tissue Location||Strongly expressed in heart, brain, liver and pancreas. Also found in lung.|
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Provided below are standard protocols that you may find useful for product applications.
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.
Jacquot, C., et al. Anticancer Res. 22(4):2229-2235(2002)
Edgar, A.J., et al. Eur. J. Biochem. 267(6):1805-1812(2000)
Tressel, T., et al. J. Biol. Chem. 261(35):16428-16437(1986)
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