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EIF3H Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - EIF3H Antibody (N-term) AP12900a
    Western blot analysis of lysate from Y79 cell line, using EIF3H Antibody (N-term) (Cat. #AP12900a). AP12900a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - EIF3H Antibody (N-term) AP12900a
    Western blot analysis of EIF3H (arrow) using rabbit polyclonal EIF3H Antibody (N-term) (Cat. #AP12900a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF3H gene.
  • IHC-P - EIF3H Antibody (N-term) AP12900a
    EIF3H Antibody (N-term) (Cat. #AP12900a)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3H Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O15372
Other Accession Q6P9U8, Q91WK2, Q5ZLE6, Q56JZ5, Q5PR67, NP_003747.1
Reactivity Human
Predicted Zebrafish, Bovine, Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39930 Da
Antigen Region 70-99 aa
Additional Information
Gene ID 8667
Other Names Eukaryotic translation initiation factor 3 subunit H {ECO:0000255|HAMAP-Rule:MF_03007}, eIF3h {ECO:0000255|HAMAP-Rule:MF_03007}, Eukaryotic translation initiation factor 3 subunit 3 {ECO:0000255|HAMAP-Rule:MF_03007}, eIF-3-gamma, eIF3 p40 subunit {ECO:0000255|HAMAP-Rule:MF_03007}, EIF3H {ECO:0000255|HAMAP-Rule:MF_03007}
Target/Specificity This EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human EIF3H.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF3H Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3H {ECO:0000255|HAMAP-Rule:MF_03007}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF- 3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03007}.
Research Areas
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Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

References

Kupfer, S.S., et al. Gastroenterology 139(5):1677-1685(2010)
Hawken, S.J., et al. Hum. Genet. 128(1):89-101(2010)
Cappuzzo, F., et al. J Thorac Oncol 4(4):472-478(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)

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$ 295.00
$ 99.00
Cat# AP12900a
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(40 western blots)
Availability: In Stock
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