|Application ||WB, IHC-P, E|
|Calculated MW||18325 Da|
|Antigen Region||119-147 aa|
|Other Names||39S ribosomal protein L50, mitochondrial, L50mt, MRP-L50, MRPL50|
|Target/Specificity||This RM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the C-terminal region of human RM50.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RM50 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)
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