|Application ||WB, IHC-P, E|
|Other Accession||Q9JMK8, NP_001005739.1|
|Calculated MW||110589 Da|
|Antigen Region||465-491 aa|
|Other Names||Vacuolar protein sorting-associated protein 54, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p, VPS54, HCC8|
|Target/Specificity||This VPS54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-491 amino acids from the Central region of human VPS54.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VPS54 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.|
|Cellular Location||Golgi apparatus, trans-Golgi network|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms
Perez-Victoria, F.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12860-12865(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009)
Meisler, M.H., et al. Amyotroph Lateral Scler 9(3):141-148(2008)
Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.