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VPS54 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - VPS54 Antibody (Center) AP12936c
    VPS54 Antibody (Center) (Cat. #AP12936c) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the VPS54 antibody detected the VPS54 protein (arrow).
  • IHC-P - VPS54 Antibody (Center) AP12936c
    VPS54 Antibody (Center) (Cat. #AP12936c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VPS54 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P1Q0
Other Accession Q9JMK8, NP_001005739.1
Reactivity Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 110589 Da
Antigen Region 465-491 aa
Additional Information
Gene ID 51542
Other Names Vacuolar protein sorting-associated protein 54, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p, VPS54, HCC8
Target/Specificity This VPS54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-491 amino acids from the Central region of human VPS54.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS54 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS54
Synonyms HCC8
Function May be involved in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
Cellular Location Golgi apparatus, trans-Golgi network
Research Areas
Citations (0)

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This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms


Perez-Victoria, F.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12860-12865(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009)
Meisler, M.H., et al. Amyotroph Lateral Scler 9(3):141-148(2008)
Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008)

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$ 295.00
$ 99.00
Cat# AP12936c
(40 western blots)
Availability: In Stock
Bulk Size
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