|Application ||WB, IHC-P, E|
|Other Accession||Q9JMK8, NP_001005739.1|
|Calculated MW||110589 Da|
|Antigen Region||465-491 aa|
|Other Names||Vacuolar protein sorting-associated protein 54, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p, VPS54, HCC8|
|Target/Specificity||This VPS54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-491 amino acids from the Central region of human VPS54.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VPS54 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).|
|Cellular Location||Golgi apparatus, trans-Golgi network|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms
Perez-Victoria, F.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12860-12865(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009)
Meisler, M.H., et al. Amyotroph Lateral Scler 9(3):141-148(2008)
Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008)
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