VPS54 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q9P1Q0 |
Other Accession | Q9JMK8, NP_001005739.1 |
Reactivity | Mouse |
Predicted | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 110589 Da |
Antigen Region | 465-491 aa |
Gene ID | 51542 |
---|---|
Other Names | Vacuolar protein sorting-associated protein 54, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p, VPS54, HCC8 |
Target/Specificity | This VPS54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-491 amino acids from the Central region of human VPS54. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | VPS54 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | VPS54 |
---|---|
Synonyms | HCC8 |
Function | Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061). |
Cellular Location | Golgi apparatus, trans-Golgi network. Membrane {ECO:0000250|UniProtKB:Q9JMK8}. Note=Associates with membranes in an EIPR1-independent manner. {ECO:0000250|UniProtKB:Q9JMK8} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms
References
Perez-Victoria, F.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12860-12865(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009)
Meisler, M.H., et al. Amyotroph Lateral Scler 9(3):141-148(2008)
Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008)
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