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DNAJC9 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DNAJC9 Antibody (N-term) AP12938A
    DNAJC9 Antibody (N-term) (Cat. #AP12938a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DNAJC9 antibody detected the DNAJC9 protein (arrow).
  • FC - DNAJC9 Antibody (N-term) AP12938A
    DNAJC9 Antibody (N-term) (Cat. #AP12938a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q8WXX5
Other Accession NP_056005.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29910 Da
Antigen Region 29-58 aa
Additional Information
Gene ID 23234
Other Names DnaJ homolog subfamily C member 9, DnaJ protein SB73, DNAJC9
Target/Specificity This DNAJC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human DNAJC9.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNAJC9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNAJC9
Function May play a role as co-chaperone of the Hsp70 family proteins HSPA1A, HSPA1B and HSPA8.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Predominantly nuclear Translocates to the cytoplasm and membrane after heat shock
Tissue Location Expressed in heart, placenta, liver, skeletal muscle, kidney, pancreas, thymus, ovary, colon and peripheral blood.
Research Areas
Citations (0)

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Background

DNAJC9 is upregulated at both mRNA and protein levels after various mitogenic and stress stimulations. DNAJC9 is mainly localized in cell nuclei under normal culture conditions, and transported into cytoplasm and plasma membrane upon heat shock stress through a non-classical and lipid-dependent pathway. DNAJC9 can interact with HSP70s and activate the ATPase activity of HSP70s, both of which are dependent on the J domain. Research has indicated that DNAJC9 is a novel cochaperone for HSP70s.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Pan, Z., et al. Neoplasma 57(2):123-128(2010)
Han, C., et al. Biochem. Biophys. Res. Commun. 353(2):280-285(2007)
Takamura, Y., et al. Biochem. Biophys. Res. Commun. 285(2):387-392(2001)

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$ 295.00
$ 99.00
Cat# AP12938A
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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