|Application ||WB, E|
|Other Accession||P50904, NP_002881.1, NP_072179.1|
|Calculated MW||116403 Da|
|Antigen Region||966-993 aa|
|Other Names||Ras GTPase-activating protein 1, GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP, RASA1, GAP, RASA|
|Target/Specificity||This RASA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 966-993 amino acids from the C-terminal region of human RASA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RASA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1.|
|Tissue Location||In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
Hemerly, J.P., et al. Eur. J. Endocrinol. 163(5):747-755(2010)
Wiemels, J.L., et al. Blood Cells Mol. Dis. 45(3):186-191(2010)
Bachas, C., et al. Blood 116(15):2752-2758(2010)
Oinuma, I., et al. J. Biol. Chem. 285(36):28200-28209(2010)
Anand, S., et al. Nat. Med. 16(8):909-914(2010)
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