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RPL35 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RPL35 Antibody (C-term) AP12959b
    RPL35 Antibody (C-term) (Cat. #AP12959b) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RPL35 antibody detected the RPL35 protein (arrow).
    detail
  • WB - RPL35 Antibody (C-term) AP12959b
    RPL35 Antibody (C-term) (Cat. #AP12959b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the RPL35 antibody detected the RPL35 protein (arrow).
    detail
  • IHC-P - RPL35 Antibody (C-term) AP12959b
    RPL35 Antibody (C-term) (Cat. #AP12959b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL35 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P42766
Other Accession Q29361, Q98TF7, Q3MHM7, NP_009140.1
Reactivity Human, Mouse
Predicted Bovine, Chicken, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14551 Da
Antigen Region 86-115 aa
Additional Information
Gene ID 11224
Other Names 60S ribosomal protein L35, RPL35
Target/Specificity This RPL35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the C-terminal region of human RPL35.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL35 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL35
Function Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:12962325, PubMed:23636399, PubMed:32669547).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

References

Chen, I.J., et al. Biochem. Cell Biol. 86(3):271-277(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)

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$ 365.00
$ 140.00
Cat# AP12959b
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