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ARFIP1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARFIP1 Antibody (Center) AP12992C
    ARFIP1 Antibody (Center) (Cat. #AP12992c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ARFIP1 antibody detected the ARFIP1 protein (arrow).
  • IHC-P - ARFIP1 Antibody (Center) AP12992C
    ARFIP1 Antibody (Center) (Cat. #AP12992c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARFIP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P53367
Other Accession Q9JHU5, NP_055262.1, NP_001020766.1
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 147-176 aa
Additional Information
Other Names Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1
Target/Specificity This ARFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the Central region of human ARFIP1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARFIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARFIP1
Function Putative target protein of ADP-ribosylation factor.
Tissue Location Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart EMBL; U52521; AAA97923.1; -; mRNA EMBL; AF124489; AAD29390.1; -; mRNA EMBL; AK290472; BAF83161.1; -; mRNA EMBL; AK299600; BAG61531.1; -; mRNA EMBL; AC099339; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC106882; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC113155; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471056; EAX04968.1; -; Genomic_DNA EMBL; CH471056; EAX04969.1; -; Genomic_DNA EMBL; BC103759; AAI03760.1; -; mRNA EMBL; BC105114; AAI05115.1; -; mRNA EMBL; BC105116; AAI05117.1; -; mRNA EMBL; BC143675; AAI43676.1; -; mRNA CCDS; CCDS34080.1; -. [P53367-1] CCDS; CCDS3780.1; -. [P53367-2] PIR; G02515; G02515 RefSeq; NP_001020764.1; NM_001025593.2. [P53367-2] RefSeq; NP_001020766.1; NM_001025595.2. [P53367-1] RefSeq; NP_001274360.1; NM_001287431.1. [P53367-1] RefSeq; NP_001274361.1; NM_001287432.1. [P53367-1] RefSeq; NP_001274362.1; NM_001287433.1. [P53367-2] RefSeq; NP_055262.1; NM_014447.3. [P53367-2] RefSeq; XP_011530171.1; XM_011531869.2. [P53367-1] RefSeq; XP_011530172.1; XM_011531870.2. [P53367-1] UniGene; Hs.416089; - ProteinModelPortal; P53367; - SMR; P53367; - BioGrid; 118084; 55 IntAct; P53367; 23 MINT; P53367; - STRING; 9606.ENSP00000296557; - iPTMnet; P53367; - PhosphoSitePlus; P53367; - DMDM; 21264399; - EPD; P53367; - MaxQB; P53367; - PaxDb; P53367; - PeptideAtlas; P53367; - PRIDE; P53367; - ProteomicsDB; 56575; - ProteomicsDB; 56576; -. [P53367-2] Ensembl; ENST00000353617; ENSP00000296557; ENSG00000164144. [P53367-1] Ensembl; ENST00000356064; ENSP00000348360; ENSG00000164144. [P53367-2] Ensembl; ENST00000405727; ENSP00000384189; ENSG00000164144. [P53367-2] Ensembl; ENST00000429148; ENSP00000396653; ENSG00000164144. [P53367-3] Ensembl; ENST00000451320; ENSP00000395083; ENSG00000164144. [P53367-1] GeneID; 27236; - KEGG; hsa:27236; - UCSC; uc003imz.5; human. [P53367-1] UCSC; uc011cij.4; human CTD; 27236; - DisGeNET; 27236; - EuPathDB; HostDB:ENSG00000164144.14; - GeneCards; ARFIP1; - HGNC; HGNC:21496; ARFIP1 HPA; CAB005080; - HPA; HPA037375; - HPA; HPA063759; - MIM; 605928; gene neXtProt; NX_P53367; - OpenTargets; ENSG00000164144; - PharmGKB; PA134920621; - eggNOG; KOG3876; Eukaryota eggNOG; ENOG410YJUM; LUCA GeneTree; ENSGT00500000044828; - HOGENOM; HOG000236308; - HOVERGEN; HBG050564; - InParanoid; P53367; - KO; K20314; - OMA; SHGFDST; - OrthoDB; EOG091G0H30; - PhylomeDB; P53367; - TreeFam; TF314945; - SIGNOR; P53367; - ChiTaRS; ARFIP1; human GeneWiki; ARFIP1; - GenomeRNAi; 27236; - PRO; PR:P53367; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000164144; - CleanEx; HS_ARFIP1; - ExpressionAtlas; P53367; baseline and differential Genevisible; P53367; HS GO; GO:0005829; C:cytosol; IDA:MGI GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB GO; GO:0032588; C:trans-Golgi network membrane; IDA:FlyBase GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:FlyBase GO; GO:0019904; F:protein domain specific binding; IEA:InterPro GO; GO:0006886; P:intracellular protein transport; IDA:MGI GO; GO:0034315; P:regulation of Arp2/3 complex-mediated actin nucleation; IBA:GO_Central GO; GO:0050708; P:regulation of protein secretion; IDA:MGI Gene3D; 1.20.1270.60; -; 1 InterPro; IPR027267; AH/BAR_dom_sf InterPro; IPR010504; AH_dom InterPro; IPR030793; Arfaptin-1 InterPro; IPR030798; Arfaptin_fam PANTHER; PTHR12141; PTHR12141; 1 PANTHER; PTHR12141:SF4; PTHR12141:SF4; 1 Pfam; PF06456; Arfaptin; 1 SMART; SM01015; Arfaptin; 1 SUPFAM; SSF103657; SSF103657; 1 PROSITE; PS50870; AH; 1 1: Evidence at protein level; Acetylation; Alternative splicing; Complete proteome; Phosphoprotein; Reference proteome INIT_MET 1 1 Removed. CHAIN 2 373 Arfaptin-1 /FTId=PRO_0000064665 DOMAIN 153 353 AH. {ECO:0000255|PROSITE- ProRule:PRU00294} MOD_RES 2 2 N-acetylalanine MOD_RES 5 5 Phosphoserine MOD_RES 28 28 Phosphoserine MOD_RES 36 36 Phosphoserine MOD_RES 39 39 Phosphoserine MOD_RES 69 69 Phosphoserine MOD_RES 79 79 Phosphoserine MOD_RES 132 132 Phosphoserine MOD_RES 361 361 Phosphothreonine VAR_SEQ 32 211 Missing (in isoform 3) /FTId=VSP_057425 VAR_SEQ 69 100 Missing (in isoform A) /FTId=VSP_004088 SEQUENCE 373 AA; 41738 MW; 7A315D0E67DECDBF CRC64; MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS HGFDNTKEGV IEAGAFQGSP APPLPSVMSP SRVAASRLAQ QGSDLIVPAG GQRTQTKSGP VILADEIKNP AMEKLELVRK WSLNTYKCTR QIISEKLGRG SRTVDLELEA QIDILRDNKK KYENILKLAQ TLSTQLFQMV HTQRQLGDAF ADLSLKSLEL HEEFGYNADT QKLLAKNGET LLGAINFFIA SVNTLVNKTI EDTLMTVKQY ESARIEYDAY RTDLEELNLG PRDANTLPKI EQSQHLFQAH KEKYDKMRND VSVKLKFLEE NKVKVLHNQL VLFHNAIAAY FAGNQKQLEQ TLKQFHIKLK TPGVDAPSWL EEQ
Research Areas
Citations (0)

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Background

ARFIP1 is a putative target protein of ADP-ribosylation factor.

References

Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Foster, L.J., et al. J. Proteome Res. 5(1):64-75(2006)
Ho, W.T., et al. FEBS Lett. 537 (1-3), 91-95 (2003) :
Suzuki, Y., et al. Genome Res. 11(5):677-684(2001)

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$ 295.00
$ 99.00
Cat# AP12992C
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(40 western blots)
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