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PIGV Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PIGV Antibody (C-term) AP13001B
    PIGV Antibody (C-term) (Cat. #AP13001b) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PIGV antibody detected the PIGV protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NUD9
Other Accession NP_060307.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55713 Da
Antigen Region 343-371 aa
Additional Information
Gene ID 55650
Other Names GPI mannosyltransferase 2, 241-, GPI mannosyltransferase II, GPI-MT-II, Phosphatidylinositol-glycan biosynthesis class V protein, PIG-V, PIGV
Target/Specificity This PIGV antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-371 amino acids from the C-terminal region of human PIGV.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIGV Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIGV
Function Alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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Background

Glycosylphosphatidylinositol (GPI) is a complex glycolipid that anchors many proteins to the cell surface. The biosynthetic pathway of GPI is mediated by sequential addition of sugars and other components to phosphatidylinositol. PIGV adds the second mannose to the GPI core (Kang et al., 2005 [PubMed 15623507]).

References

Krawitz, P.M., et al. Nat. Genet. 42(10):827-829(2010)
Kang, J.Y., et al. J. Biol. Chem. 280(10):9489-9497(2005)
Fabre, A.L., et al. FEBS J. 272(5):1160-1168(2005)
Kinoshita, T., et al. Curr Opin Chem Biol 4(6):632-638(2000)

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$ 365.00
$ 140.00
Cat# AP13001B
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