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Bak Antibody (BH3 Domain Specific)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP1301a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Bak Antibody (BH3 Domain Specific) - Product info | |
| Application | IHC, WB
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| Primary Accession | Q16611 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 23409 Da |
Bak Antibody (BH3 Domain Specific) - Additional info | |
| Gene ID 578 | |
| Other Names BAK1; BAK; BCL2L7; CDN1; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7 | |
| Target/Specificity This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66~96 amino acids from the N-terminal region (BH3 domain) of human Bak. | |
| Dilution IHC~~1:50~100 WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Bak Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Bak Antibody (BH3 Domain Specific) - Protein Information | |
| Name BAK1 | |
| Synonyms BAK, BCL2L7, CDN1 | |
| Function In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis | |
| Cellular Location Mitochondrion membrane; Single-pass membrane protein (Potential) | |
| Tissue Location Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle | |
Bak Antibody (BH3 Domain Specific) - Related products
AP1301a: Bak Antibody (BH3 Domain Specific)
DC01487: Human BAK1 cDNA Clone
LY11623a: BAK1 Over-expression Lysate
Bak Antibody (BH3 Domain Specific) - Application data
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Bak Antibody (BH3) (Cat. #AP1301a) western blot analysis in Jurkat,MDA-MB453,293 cell line lysates (35ug/lane).This demonstrates the Bak antibody detected the Bak protein (arrow).
Bak Antibody (BH3 Domain Specific) - Research Areas
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BACKGROUND
BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
REFERENCES
Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003). Mikhailov, V., et al., J. Biol. Chem. 278(7):5367-5376 (2003). Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002). Bellosillo, B., et al., Blood 100(5):1810-1816 (2002). Grutkoski, P.S., et al., Shock 17(1):47-54 (2002).
