|Application ||WB, IHC-P, E|
|Calculated MW||51546 Da|
|Antigen Region||387-415 aa|
|Other Names||Abscission/NoCut checkpoint regulator, ANCHR, MLL partner containing FYVE domain, Zinc finger FYVE domain-containing protein 19, ZFYVE19, ANCHR, MPFYVE|
|Target/Specificity||This ZFYVE19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human ZFYVE19.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZFYVE19 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cleavage furrow. Midbody. Note=Localizes mainly on centrosomes in interphase and early mitosis. Localizes at the cleavage furrow and midbody ring in late mitosis and cytokinesis (PubMed:24814515).|
|Tissue Location||Detected in brain, heart, skeletal muscle, kidney and liver.|
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Provided below are standard protocols that you may find useful for product applications.
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Chinwalla, V., et al. Oncogene 22(9):1400-1410(2003)
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