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Bcl-2 Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Anti-Bcl- 2 Antibody (BH3) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Anti-Bcl-2-BH3 Antibody at 1:1000 dilution + PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26.2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (Cat#AP1303a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP1303a antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using AP1303a in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10415
Other Accession P49950, O02718
Reactivity Human, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 26266 Da
Antigen Region 75-110 aa
Additional Information
Gene ID 596
Other Names Apoptosis regulator Bcl-2, BCL2
Target/Specificity This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-2 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Expressed in a variety of tissues.
Research Areas
Citations ( 0 )


BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.


Zhu, C.J., et al., J. Neurosci. Res. 74(1):60-66 (2003). Scopa, C.D., et al., Dig. Dis. Sci. 48(10):1990-1997 (2003). Grace, V.M., et al., Gynecol. Oncol. 91(1):51-58 (2003). Thoulouze, M.I., et al., Virology 314(2):549-561 (2003). Iwata, A., et al., J. Immunol. 171(6):3136-3141 (2003).

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$ 295.00
$ 99.00
Cat# AP1303a
(40 western blots)
Availability: In Stock
Bulk Size
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