|Application ||WB, E|
|Other Accession||Q32LH4, NP_001172023.1, NP_005591.1, NP_001172022.1, NP_001172021.1|
|Calculated MW||35896 Da|
|Antigen Region||298-327 aa|
|Other Names||Nitrilase homolog 1, 35--, NIT1|
|Target/Specificity||This NIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the C-terminal region of human NIT1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NIT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity).|
|Cellular Location||Cytoplasm. Mitochondrion.|
|Tissue Location||Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.
Semba, S., et al. J. Biol. Chem. 281(38):28244-28253(2006)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Pekarsky, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 95(15):8744-8749(1998)
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