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NIT1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NIT1 Antibody (C-term) AP13042b
    NIT1 Antibody (C-term) (Cat. #AP13042b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the NIT1 antibody detected the NIT1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86X76
Other Accession Q32LH4, NP_001172023.1, NP_005591.1, NP_001172022.1, NP_001172021.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35896 Da
Antigen Region 298-327 aa
Additional Information
Gene ID 4817
Other Names Nitrilase homolog 1, 35--, NIT1
Target/Specificity This NIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the C-terminal region of human NIT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNIT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NIT1
Function Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity).
Cellular Location Cytoplasm. Mitochondrion.
Tissue Location Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.


Semba, S., et al. J. Biol. Chem. 281(38):28244-28253(2006)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Pekarsky, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 95(15):8744-8749(1998)

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$ 295.00
$ 99.00
Cat# AP13042b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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