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HIST1H2AB Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HIST1H2AB Antibody (N-term) AP13062a
    HIST1H2AB Antibody (N-term) (Cat. #AP13062a) western blot analysis in K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
  • IHC-P - HIST1H2AB Antibody (N-term) AP13062a
    HIST1H2AB Antibody (N-term) (Cat. #AP13062a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HIST1H2AB Antibody (N-term) AP13062a
    HIST1H2AB Antibody (N-term) (Cat. #AP13062a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04908
Other Accession Q6GM86, Q7ZUY3, A9UMV8, Q8R1M2, Q4R3X5, Q9BTM1, P70082, Q3ZBX9, P02263, Q4FZT6, Q8BFU2, Q7L7L0, P35062, P06898, Q64523, Q16777, A1A4R1, Q64522, Q8IUE6, P0CC09, Q6GSS7, Q6FI13, P06897, P02262, P22752, P0C0S8, P0C0S9, Q8CGP7, Q99878, Q8CGP6, Q96KK5, Q64598
Reactivity Human
Predicted Rat, Mouse, Bovine, Xenopus, Chicken, Monkey, Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14135 Da
Antigen Region 12-40 aa
Additional Information
Gene ID 3012;8335
Other Names Histone H2A type 1-B/E, Histone H2A2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM
Target/Specificity This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human HIST1H2AB.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H2AB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H2AFM
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.


Rose, J. Phd, et al. Mol. Med. (2010) In press :
Bergink, S., et al. Genes Dev. 20(10):1343-1352(2006)
Cao, R., et al. Mol. Cell 20(6):845-854(2005)
Hagiwara, T., et al. Biochemistry 44(15):5827-5834(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)

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$ 295.00
$ 99.00
Cat# AP13062a
(40 western blots)
Availability: In Stock
Bulk Size
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