|Application ||WB, IHC-P, FC, E|
|Other Accession||Q6GM86, Q7ZUY3, A9UMV8, Q8R1M2, Q4R3X5, Q9BTM1, P70082, Q3ZBX9, P02263, Q4FZT6, Q8BFU2, Q7L7L0, P35062, P06898, Q64523, Q16777, A1A4R1, Q64522, Q8IUE6, P0CC09, Q6GSS7, Q6FI13, P06897, P02262, P22752, P0C0S8, P0C0S9, Q8CGP7, Q99878, Q8CGP6, Q96KK5, Q64598|
|Predicted||Mouse, Rat, Bovine, Xenopus, Chicken, Monkey, Zebrafish|
|Calculated MW||14135 Da|
|Antigen Region||12-40 aa|
|Other Names||Histone H2A type 1-B/E, Histone H2A2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM|
|Target/Specificity||This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human HIST1H2AB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIST1H2AB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|Cellular Location||Nucleus. Chromosome.|
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Bergink, S., et al. Genes Dev. 20(10):1343-1352(2006)
Cao, R., et al. Mol. Cell 20(6):845-854(2005)
Hagiwara, T., et al. Biochemistry 44(15):5827-5834(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
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