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GNAS Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GNAS Antibody (C-term) AP13065b
    Western blot analysis of GNAS (arrow) using rabbit polyclonal GNAS Antibody (C-term) (Cat. #AP13065b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.
  • WB - GNAS Antibody (C-term) AP13065b
    GNAS Antibody (C-term) (Cat. #AP13065b) western blot analysis in 293,NCI-H292 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow).
  • IHC-P - GNAS Antibody (C-term) AP13065b
    GNAS Antibody (C-term) (Cat. #AP13065b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GNAS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - GNAS Antibody (C-term) AP13065b
    Confocal immunofluorescent analysis of GNAS Antibody (C-term)(Cat#AP13065b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - GNAS Antibody (C-term) AP13065b
    GNAS Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5FWY2
Other Accession P29797, Q8R4A8, P63095, P63094, P63092, P04896, Q63803, Q6R0H7, Q5JWF2
Reactivity Human
Predicted Mouse, Rat, Bovine, Hamster, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44250 Da
Antigen Region 287-315 aa
Additional Information
Gene ID 2778
Other Names GNAS
Target/Specificity This GNAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-315 amino acids from the C-terminal region of human GNAS.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNAS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNAS {ECO:0000313|EMBL:AAH89157.2}
Citations (0)

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Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

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$ 295.00
$ 99.00
Cat# AP13065b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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