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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MPP3 Antibody (C-term)   

MPP3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MPP3 Antibody (C-term) AP13068b
    Anti-MPP3 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MPP3 Antibody (C-term) AP13068b
    Anti-MPP3 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MPP3 Antibody (C-term) AP13068b
    MPP3 Antibody (C-term) (Cat. #AP13068b) western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPP3 antibody detected the MPP3 protein (arrow).
  • IF - MPP3 Antibody (C-term) AP13068b
    Confocal immunofluorescent analysis of MPP3 Antibody (C-term)(Cat#AP13068b) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - MPP3 Antibody (C-term) AP13068b
    MPP3 Antibody (C-term) (Cat. #AP13068b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession Q13368
Other Accession NP_001923.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 66152 Da
Antigen Region 406-434 aa
Additional Information
Gene ID 4356
Other Names MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3, MPP3, DLG3
Target/Specificity This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3.
Dilution WB~~1:1000
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMPP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MPP3
Synonyms DLG3
Research Areas
Citations (0)

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Background

This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.

References

Qin, L., et al. Zhonghua Gan Zang Bing Za Zhi 15(7):509-512(2007)
Kantardzhieva, A., et al. FEBS J. 273(6):1152-1165(2006)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Fukuhara, H., et al. Oncogene 22(40):6160-6165(2003)
Shin, B.K., et al. J. Biol. Chem. 278(9):7607-7616(2003)

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$ 295.00
$ 99.00
Cat# AP13068b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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