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mouse BID Antibody (S61)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - mouse BID Antibody (S61) AP1307d
    Western blot analysis of anti-mouse BID Antibody (S61) (Cat.#AP1307d) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab .
  • IHC-P - mouse BID Antibody (S61) AP1307d
    mouse BID Antibody (S61)(Cat.#AP1307d) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - mouse BID Antibody (S61) AP1307d
    mouse BID Antibody (S61) (Cat.#AP1307d) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P70444
Other Accession NP_031570
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21952 Da
Antigen Region 39-68 aa
Additional Information
Gene ID 12122
Other Names BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, Bid
Target/Specificity This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from mouse BID.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsmouse BID Antibody (S61) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Bid
Function Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.
Cellular Location Cytoplasm. Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane
Research Areas
Citations (0)

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Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.


Ziporen,L., J. Immunol. 182 (1), 515-521 (2009)
Kaufmann,T., Immunity 30 (1), 56-66 (2009)
Mandal,M., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20840-20845 (2008)

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$ 295.00
$ 99.00
Cat# AP1307d
(40 western blots)
Availability: In Stock
Bulk Size
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