|Application ||WB, E|
|Calculated MW||50283 Da|
|Antigen Region||227-256 aa|
|Other Names||Origin recognition complex subunit 5, ORC5, ORC5L|
|Target/Specificity||This ORC5L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 amino acids from the Central region of human ORC5L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ORC5L Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre- replication complex necessary to initiate DNA replication.|
|Tissue Location||Abundant in spleen, ovary, prostate, testis, and colon mucosa.|
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Provided below are standard protocols that you may find useful for product applications.
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Trynka, G., et al. Gut 58(8):1078-1083(2009)
Siddiqui, K., et al. J. Biol. Chem. 282(44):32370-32383(2007)
Clarke, C.A., et al. Biochem. J. 388 (PT 2), 705-712 (2005) :
Volkening, M., et al. Mol. Cell. Biol. 25(4):1560-1568(2005)
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