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MCL1 Antibody (BH3 Domain Specific)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1312a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP1312a-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
MCL1 Antibody (BH3 Domain Specific) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | Q07820 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 37337 Da |
MCL1 Antibody (BH3 Domain Specific) - Additional info | |
| Gene ID 4170 | |
| Other Names MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT | |
| Target/Specificity This MCL1 antibody was developed against a synthetic peptide selected from the central region (BH3 domain) of human Mcl-1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MCL1 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MCL1 Antibody (BH3 Domain Specific) - Protein Information | |
| Name MCL1 | |
| Synonyms BCL2L3 | |
| Function Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis Isoform 2 promotes apoptosis | |
| Cellular Location Membrane; Single-pass membrane protein (Potential). Cytoplasm. Mitochondrion. Nucleus, nucleoplasm Note=Cytoplasmic, associated with mitochondria | |
MCL1 Antibody (BH3 Domain Specific) - Related products
AP1312a: MCL1 Antibody (BH3 Domain Specific)
MCL1 Antibody (BH3 Domain Specific) - Application data
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Western blot analysis of anti-Mcl-1 BH3 Domain Pab (Cat. #AP1312a) in Ramos cell line lysates (35ug/lane). Mcl-1-BH3(arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of ZR-75-1 cells using MCL1 Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MCL1 Antibody (BH3 Domain Specific) - Research Areas
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BACKGROUND
The Mcl-1 protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
REFERENCES
Crossley, L.J., J. Leukoc. Biol. 74(4):583-592 (2003). Kotelkin, A., et al., J. Virol. 77(17):9156-9172 (2003). Erwert, R.D., et al., Microb. Pathog. 35(2):87-93 (2003). Liu, H., et al., Blood 102(1):344-352 (2003). Nijhawan, D., et al., Genes Dev. 17(12):1475-1486 (2003).
