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Bad Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Bad Antibody (Center) AP1314C
    Anti-Bad Antibody at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Bad Antibody (Center) AP1314C
    Anti-Bad Antibody at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Bad Antibody (Center) AP1314C
    Western blot analysis of anti-Bad Antibody (Center)(Cat.#AP1314c) in HL60 cell line lysates (35ug/lane). Bad(arrow) was detected using the purified Pab.
  • IHC-P - Bad Antibody (Center) AP1314C
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bad antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q92934
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 18392 Da
Antigen Region 53-81 aa
Additional Information
Gene ID 572
Other Names Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BAD, BBC6, BCL2L8
Target/Specificity This Bad antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-81 amino acids from the Central region of human Bad.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBad Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BAD
Synonyms BBC6, BCL2L8
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Cellular Location Mitochondrion outer membrane. Cytoplasm {ECO:0000250|UniProtKB:Q61337}. Note=Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm. {ECO:0000250|UniProtKB:Q61337}
Tissue Location Expressed in a wide variety of tissues.
Research Areas
Citations ( 0 )

Background

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein.

References

Lee, J.W., et al., Carcinogenesis 25(8):1371-1376 (2004).
Zhang, B., et al., Mol. Cell. Biol. 24(14):6205-6214 (2004).
Ong, C.S., et al., Oncol. Rep. 11(3):727-733 (2004).
Yan, B., et al., J. Biol. Chem. 278(46):45358-45367 (2003).
Taghiyev, A.F., et al., Mol. Cancer Res. 1(7):500-507 (2003).

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$ 295.00
$ 99.00
Cat# AP1314C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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