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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AMELX Antibody (N-term)   

AMELX Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AMELX Antibody (N-term) AP13169A
    AMELX Antibody (N-term) (Cat. #AP13169a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AMELX antibody detected the AMELX protein (arrow).
  • IHC-P - AMELX Antibody (N-term) AP13169A
    AMELX Antibody (N-term) (Cat. #AP13169a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AMELX Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - AMELX Antibody (N-term) AP13169A
    AMELX Antibody (N-term) (Cat. #AP13169a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q99217
Other Accession NP_872621.1, NP_001133.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21603 Da
Antigen Region 17-46 aa
Additional Information
Gene ID 265
Other Names Amelogenin, X isoform, AMELX, AMG, AMGX
Target/Specificity This AMELX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human AMELX.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMELX Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMELX
Synonyms AMG, AMGX
Function Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)

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Background

This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Almqvist, S., et al. J Mater Sci Mater Med 21(3):947-954(2010)
Lee, S.K., et al. J. Dent. Res. 89(1):46-50(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Tomas, C., et al. Forensic Sci Int Genet 3(1):1-6(2008)

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Cat# AP13169A
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(40 western blots)
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