- CITATIONS: 1
|Application ||WB, IHC-P, IF, FC, E|
|Other Accession||Q80ZG6, Q99ML1|
|Calculated MW||20532 Da|
|Antigen Region||119-154 aa|
|Other Names||Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA|
|Target/Specificity||This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Puma BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.|
|Cellular Location||Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release|
|Tissue Location||Ubiquitously expressed.|
Provided below are standard protocols that you may find useful for product applications.
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Hoque, M.O., et al., Cancer Lett. 199(1):75-81 (2003). Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(4):1931-1936 (2003). Han, J., et al., Proc. Natl. Acad. Sci. U.S.A. 98(20):11318-11323 (2001). Nakano, K., et al., Mol. Cell 7(3):683-694 (2001).
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