- CITATIONS: 1
|Application ||WB, IHC-P, IF, FC, E|
|Other Accession||Q80ZG6, Q99ML1|
|Calculated MW||20532 Da|
|Antigen Region||119-154 aa|
|Other Names||Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA|
|Target/Specificity||This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Puma BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.|
|Cellular Location||Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release|
|Tissue Location||Ubiquitously expressed.|
Provided below are standard protocols that you may find useful for product applications.
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Hoque, M.O., et al., Cancer Lett. 199(1):75-81 (2003). Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(4):1931-1936 (2003). Han, J., et al., Proc. Natl. Acad. Sci. U.S.A. 98(20):11318-11323 (2001). Nakano, K., et al., Mol. Cell 7(3):683-694 (2001).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.