|Application ||WB, E|
|Calculated MW||82803 Da|
|Antigen Region||685-714 aa|
|Other Names||Poly(A) polymerase gamma, PAP-gamma, Neo-poly(A) polymerase, Neo-PAP, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme, PAPOLG, PAP2, PAPG|
|Target/Specificity||This PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-714 amino acids from the C-terminal region of human PAPOLG.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PAPOLG Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.|
|Tissue Location||Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.
Lee, Y.S., et al. J. Biol. Chem. 285(36):28105-28116(2010)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Kyriakopoulou, C.B., et al. J. Biol. Chem. 276(36):33504-33511(2001)
Topalian, S.L., et al. Mol. Cell. Biol. 21(16):5614-5623(2001)
Perumal, K., et al. J. Biol. Chem. 276(24):21791-21796(2001)
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