- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Calculated MW||18016 Da|
|Antigen Region||39-74 aa|
|Other Names||Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK|
|Target/Specificity||This Bik BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-74 amino acids from human Bik BH3 Domain.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bik BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.|
|Cellular Location||Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes|
Provided below are standard protocols that you may find useful for product applications.
The Bik protein is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.
Arena, V., et al., Genes Chromosomes Cancer 38(1):91-96 (2003).
Gillissen, B., et al., EMBO J. 22(14):3580-3590 (2003).
Germain, M., et al., J. Biol. Chem. 277(20):18053-18060 (2002).
Zou, Y., et al., Cancer Res. 62(1):8-12 (2002).
Castells, A., et al., Gastroenterology 117(4):831-837 (1999).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.