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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NDST1 Antibody (C-term)   

NDST1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NDST1 Antibody (C-term) AP13224b
    NDST1 Antibody (C-term) (Cat. #AP13224b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow).
  • IHC-P - NDST1 Antibody (C-term) AP13224b
    NDST1 Antibody (C-term) (Cat. #AP13224b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDST1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P52848
Other Accession Q02353, Q3UHN9, NP_001534.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 100868 Da
Antigen Region 820-849 aa
Additional Information
Gene ID 3340
Other Names Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, NDST-1, N-heparan sulfate sulfotransferase 1, N-HSST 1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, HSNST 1, Heparan sulfate N-deacetylase 1, 3---, Heparan sulfate N-sulfotransferase 1, 282-, NDST1, HSST, HSST1
Target/Specificity This NDST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-849 amino acids from the C-terminal region of human NDST1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDST1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDST1
Synonyms HSST, HSST1
Function Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Widely expressed. Expression is most abundant in heart, liver and pancreas
Research Areas
Citations (0)

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Background

NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.

References

Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Duelli, A., et al. J. Immunol. 183(11):7073-7083(2009)
Zuberi, R.I., et al. J. Immunol. 183(6):3971-3979(2009)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)

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$ 295.00
$ 99.00
Cat# AP13224b
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(40 western blots)
Availability: In Stock
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