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SGPP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SGPP1 Antibody (N-term) AP13228a
    Anti-SGPP1 Antibody (N-term) at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SGPP1 Antibody (N-term) AP13228a
    SGPP1 Antibody (N-term) (Cat. #AP13228a) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SGPP1 antibody detected the SGPP1 protein (arrow).
  • IHC-P - SGPP1 Antibody (N-term) AP13228a
    SGPP1 Antibody (N-term) (Cat. #AP13228a)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGPP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BX95
Other Accession NP_110418.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49108 Da
Antigen Region 13-42 aa
Additional Information
Gene ID 81537
Other Names Sphingosine-1-phosphate phosphatase 1, SPPase1, Spp1, hSPP1, hSPPase1, 313-, Sphingosine-1-phosphatase 1, SGPP1
Target/Specificity This SGPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SGPP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSGPP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SGPP1
Function Has enzymatic activity against both sphingosine 1- phosphate (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P levels.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Ubiquitous, with the strongest level in placenta and kidney.
Research Areas
Citations (0)

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Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM].


Hicks, A.A., et al. PLoS Genet. 5 (10), E1000672 (2009) :
Le Stunff, H., et al. J. Biol. Chem. 282(47):34372-34380(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Giussani, P., et al. Mol. Cell. Biol. 26(13):5055-5069(2006)

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$ 295.00
$ 99.00
Cat# AP13228a
(40 western blots)
Availability: In Stock
Bulk Size
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