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PGAP3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PGAP3 Antibody (Center) AP13237c
    PGAP3 Antibody (Center) (Cat. #AP13237c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PGAP3 antibody detected the PGAP3 protein (arrow).
  • IHC-P - PGAP3 Antibody (Center) AP13237c
    PGAP3 Antibody (Center) (Cat. #AP13237c)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PGAP3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96FM1
Other Accession A2A559, A2V7M9, A7YWP2, NP_219487.3
Reactivity Human
Predicted Bovine, Hamster, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36475 Da
Antigen Region 141-169 aa
Additional Information
Gene ID 93210
Other Names Post-GPI attachment to proteins factor 3, COS16 homolog, hCOS16, Gene coamplified with ERBB2 protein, PER1-like domain-containing protein 1, PGAP3, CAB2, PERLD1
Target/Specificity This PGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-169 amino acids from the Central region of human PGAP3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGAP3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGAP3
Synonyms CAB2, PERLD1
Function Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable).
Cellular Location Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Mainly localizes to Golgi apparatus.
Tissue Location Ubiquitously expressed, with highest levels in thyroid and placenta.
Citations (0)

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Background

PGAP3 is involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable).

References

Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009)
Maeda, Y., et al. Mol. Biol. Cell 18(4):1497-1506(2007)
Benusiglio, P.R., et al. Br. J. Cancer 95(12):1689-1695(2006)

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Cat# AP13237c
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(40 western blots)
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