|Application ||WB, E|
|Calculated MW||50381 Da|
|Antigen Region||361-390 aa|
|Other Names||Synaptonemal complex protein SC65, Leprecan-like protein 4, Nucleolar autoantigen No55, LEPREL4, NOL55, SC65|
|Target/Specificity||This SC65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 amino acids from the C-terminal region of human SC65.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SC65 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Nucleus, nucleolus. Chromosome. Note=Localized uniformly throughout the granular component of the nucleolus and on the surface of chromosomes during mitosis|
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Provided below are standard protocols that you may find useful for product applications.
This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes.
Foster, L.J., et al. J. Proteome Res. 5(1):64-75(2006)
Fossa, A., et al. Br. J. Cancer 83(6):743-749(2000)
Ochs, R.L., et al. Mol. Biol. Cell 7(7):1015-1024(1996)
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