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Bit1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Bit1 Antibody (Center) AP1323a
    Bit1 Antibody (K95) (Cat. #AP1323a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Bit1 antibody detected the Bit1 protein (arrow).
  • IHC-P - Bit1 Antibody (Center) AP1323a
    Bit1 Antibody (Center) (Cat. #AP1323a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Bit1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y3E5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 19194 Da
Antigen Region 80-110 aa
Additional Information
Gene ID 51651
Other Names Peptidyl-tRNA hydrolase 2, mitochondrial, PTH 2, Bcl-2 inhibitor of transcription 1, PTRH2, BIT1, PTH2
Target/Specificity This Bit1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the Central region of human Bit1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBit1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTRH2
Synonyms BIT1, PTH2
Function The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis.
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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Adhesion to extracellular matrix regulates cell survival via integrin engagement and cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. A role for Bit1 (Bcl-2 inhibitor of transcription 1) has been identified in this process. Bit1 is a mitochondrial protein released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. Bit1-AES complexes may switch off a survival-promoting gene transcription program controlled by TLE. Apoptosis of Bit1/AES transfected cells is inhibited when cells are permitted to attach to fibronectin through the alpha-beta integrin, suggesting that the contribution of the Bit1-AES pathway to anoikis is regulated by integrins.


Cell 116(5):751-762 (2004).
Biochim Biophys Acta. 1692:145-57 (2004).
Gene 2000; 249:1-16.

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$ 295.00
$ 99.00
Cat# AP1323a
(40 western blots)
Availability: In Stock
Bulk Size
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