|Application ||WB, E|
|Other Accession||NP_001011645.1, NP_000035.2|
|Calculated MW||99188 Da|
|Antigen Region||475-504 aa|
|Other Names||Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4|
|Target/Specificity||This AR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human AR.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.|
|Cellular Location||Nucleus Cytoplasm Note=Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1|
|Tissue Location||Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).|
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Provided below are standard protocols that you may find useful for product applications.
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq].
Shu, S.K., et al. J. Biol. Chem. 285(43):33045-33053(2010)
Nedelsky, N.B., et al. Neuron 67(6):936-952(2010)
Panda, B., et al. Gynecol. Endocrinol. (2010) In press :
Schneider, G., et al. Am J Geriatr Psychiatry (2010) In press :
Guadalupe-Grau, A., et al. PLoS ONE 5 (7), E11529 (2010) :
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