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CASP10 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Alternative | |||
| AP1326b | AP1326c | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | ||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CASP10 Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | Q92851 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 58951 Da |
CASP10 Antibody (C-term) - Additional info | |
| Gene ID 843 | |
| Other Names CASP10; MCH4; Caspase-10; Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; ICE-like apoptotic protease 4; Caspase-10 subunit p23/17; Caspase-10 subunit p12 | |
| Target/Specificity This CASP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467~497 amino acids from the C-terminal region of human CASP10. | |
| Dilution WB~~1ÂF50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CASP10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CASP10 Antibody (C-term) - Protein Information | |
| Name CASP10 | |
| Synonyms MCH4 | |
| Function Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC | |
| Tissue Location Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon | |
CASP10 Antibody (C-term) - Related products
AP1326b: CASP10 Antibody (C-term)
AP1326c: CASP10 Antibody (Center)
RI10796: CASP10 predesign siRNA
DC07501: Human CASP10 cDNA Clone
BP1326b: CASP10 Antibody (C-term) Blocking Peptide
BP1326c: CASP10 Antibody (Center) Blocking Peptide
AT1401a: CASP10 Antibody (monoclonal) (M01)
CASP10 Antibody (C-term) - Application data
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Western blot analysis of CASP10 antibody (C-term) (Cat.#AP1326b) in K562 cell line lysates (35ug/lane). CASP10 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with CASP10 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of HepG2 cells using CASP10 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CASP10 Antibody (C-term) - Research Areas
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BACKGROUND
CASP10 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in the protein are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome.
REFERENCES
Lan,Q., Morton,L.M. Blood 114 (2), 264-267 (2009) Kim,Y.R., Kim,K.M. Hum. Pathol. 40 (6), 868-871 (2009) Lisa-Santamaria,P. Biochim. Biophys. Acta 1793 (3), 561-571 (2009)
