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TRIM4 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TRIM4 Antibody (N-term) AP13289a
    TRIM4 Antibody (N-term) (Cat. #AP13289a) western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).This demonstrates the TRIM4 antibody detected the TRIM4 protein (arrow).
    detail
  • IHC-P - TRIM4 Antibody (N-term) AP13289a
    TRIM4 Antibody (N-term) (Cat. #AP13289a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9C037
Other Accession NP_148977.2, NP_149082.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57461 Da
Antigen Region 112-141 aa
Additional Information
Gene ID 89122
Other Names E3 ubiquitin-protein ligase TRIM4, 632-, RING finger protein 87, Tripartite motif-containing protein 4, TRIM4, RNF87
Target/Specificity This TRIM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-141 amino acids from the N-terminal region of human TRIM4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIM4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM4
Synonyms RNF87
Function E3 ubiquitin-protein ligase. Mediates 'Lys-63'-linked polyubiquitination of the innate immune receptor RIGI, this linkage doesn't lead to proteasomal degradation but seems to enhance IFN induction.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.

References

Kasyapa, C., et al. Proteomics 9(16):3979-3988(2009)
Li, X., et al. Virology 360(2):419-433(2007)
Thompson, E.E., et al. Pharmacogenomics J. 6(2):105-114(2006)
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)

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$ 365.00
$ 140.00
Cat# AP13289a
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