|Application ||WB, IHC-P, E|
|Other Accession||Q6P6W3, O88895, NP_003874.2|
|Calculated MW||48848 Da|
|Antigen Region||383-412 aa|
|Other Names||Histone deacetylase 3, HD3, RPD3-2, SMAP45, HDAC3|
|Target/Specificity||This HDAC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-412 amino acids from the C-terminal region of human HDAC3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HDAC3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys- 27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed:21444723, PubMed:23911289). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803).|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, cytosol. Note=Colocalizes with XBP1 and AKT1 in the cytoplasm (PubMed:25190803). Predominantly expressed in the nucleus in the presence of CCAR2.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq].
Minamiya, Y., et al. Tumour Biol. 31(5):533-539(2010)
Kim, H.C., et al. Cell. Mol. Life Sci. 67(20):3499-3510(2010)
Yang, Z., et al. Clin. Chem. Lab. Med. (2010) In press :
Kim, T., et al. Psychiatry Res 178(2):266-269(2010)
Adams, H., et al. Expert Opin. Ther. Targets 14(6):577-584(2010)
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