PIK3R4 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q99570 |
Other Accession | NP_055417.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 153103 Da |
Antigen Region | 819-847 aa |
Gene ID | 30849 |
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Other Names | Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase regulatory subunit 4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase adaptor protein, PIK3R4 |
Target/Specificity | This PIK3R4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-847 amino acids from the Central region of human PIK3R4. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PIK3R4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PIK3R4 |
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Synonyms | VPS15 {ECO:0000303|PubMed:23878393} |
Function | Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). |
Cellular Location | Late endosome. Cytoplasmic vesicle, autophagosome. Membrane; Lipid-anchor. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity) {ECO:0000250|UniProtKB:Q8VD65, ECO:0000305} |
Tissue Location | Ubiquitously expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
References
Matsunaga, K., et al. Nat. Cell Biol. 11(4):385-396(2009)
Yan, Y., et al. Biochem. J. 417(3):747-755(2009)
Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004)
Takeshita, F., et al. Semin. Immunol. 16(1):17-22(2004)
Ligon, L.A., et al. Mol. Biol. Cell 14(4):1405-1417(2003)
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