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PI15 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PI15 Antibody (N-term) AP13330a
    All lanes : Anti-PI15 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PI15 Antibody (N-term) AP13330a
    PI15 Antibody (N-term) (Cat. #AP13330a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).
  • IHC-P - PI15 Antibody (N-term) AP13330a
    PI15 Antibody (N-term) (Cat. #AP13330a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PI15 Antibody (N-term) AP13330a
    PI15 Antibody (N-term) (Cat. #AP13330a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43692
Other Accession NP_056970.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29065 Da
Antigen Region 34-63 aa
Additional Information
Gene ID 51050
Other Names Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI
Target/Specificity This PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human PI15.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PI15
Synonyms CRISP8, P25TI
Function Serine protease inhibitor which displays weak inhibitory activity against trypsin.
Cellular Location Secreted.
Tissue Location Weakly expressed. Expressed at low level in prostate, mammary gland, salivary gland and thyroid gland
Research Areas
Citations (0)

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This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq].


Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :
Kaplan, F., et al. Am. J. Physiol. 276 (6 PT 1), L1027-L1036 (1999) :
Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)
Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)
Koshikawa, N., et al. J. Biochem. 119(2):334-339(1996)

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$ 295.00
$ 99.00
Cat# AP13330a
(40 western blots)
Availability: In Stock
Bulk Size
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