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ROBLD3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ROBLD3 Antibody (Center) AP13338c
    ROBLD3 Antibody (Center) (Cat. #AP13338c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ROBLD3 antibody detected the ROBLD3 protein (arrow).
  • IHC-P - ROBLD3 Antibody (Center) AP13338c
    ROBLD3 Antibody (Center) (Cat. #AP13338c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROBLD3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y2Q5
Other Accession Q3T132, NP_054736.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13508 Da
Antigen Region 41-70 aa
Additional Information
Gene ID 28956
Other Names Ragulator complex protein LAMTOR2, Endosomal adaptor protein p14, Late endosomal/lysosomal Mp1-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, Mitogen-activated protein-binding protein-interacting protein, MAPBP-interacting protein, Roadblock domain-containing protein 3, LAMTOR2, MAPBPIP, ROBLD3
Target/Specificity This ROBLD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the Central region of human ROBLD3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsROBLD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LAMTOR2
Synonyms MAPBPIP, ROBLD3
Function As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
Cellular Location Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side
Research Areas
Citations (0)

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Background

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

References

Sancak, Y., et al. Cell 141(2):290-303(2010)
Wunderlich, W., et al. J. Cell Biol. 152(4):765-776(2001)

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$ 295.00
$ 99.00
Cat# AP13338c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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