|Application ||WB, IHC-P, E|
|Other Accession||Q3T132, NP_054736.1|
|Calculated MW||13508 Da|
|Antigen Region||41-70 aa|
|Other Names||Ragulator complex protein LAMTOR2, Endosomal adaptor protein p14, Late endosomal/lysosomal Mp1-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, Mitogen-activated protein-binding protein-interacting protein, MAPBP-interacting protein, Roadblock domain-containing protein 3, LAMTOR2, MAPBPIP, ROBLD3|
|Target/Specificity||This ROBLD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the Central region of human ROBLD3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ROBLD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.|
|Cellular Location||Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Sancak, Y., et al. Cell 141(2):290-303(2010)
Wunderlich, W., et al. J. Cell Biol. 152(4):765-776(2001)
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