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HSPA5 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HSPA5 Antibody AP1335A
    The anti-HSPA5 Pab (Cat. #AP1335a) is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.
  • WB - HSPA5 Antibody AP1335A
    Western blot analysis of anti-HSPA5 Pab (Cat. #AP1335a) in HL60 cell line lysates (35ug/lane).HSPA5(arrow) was detected using the purified Pab.
  • IHC-P - HSPA5 Antibody AP1335A
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody (Cat.#AP1335a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - HSPA5 Antibody AP1335A
    Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody (Cat.#AP1335a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - HSPA5 Antibody AP1335A
    Confocal immunofluorescent analysis of HSPA5 Antibody(Cat#AP1335a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - HSPA5 Antibody AP1335A
    HSPA5 Antibody (Cat. #AP1335a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11021
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 72333 Da
Additional Information
Gene ID 3309
Other Names 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Target/Specificity This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA5
Synonyms GRP78
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations ( 0 )


In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation. They are involved in signal transduction pathways in cooperation with HSP90. They participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.


Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Milner, C.M., et al., Immunogenetics 32(4):242-251 (1990). Sargent, C.A., et al., Proc. Natl. Acad. Sci. U.S.A. 86(6):1968-1972 (1989). Drabent, B., et al., Nucleic Acids Res. 14(22):8933-8948 (1986). Hunt, C., et al., Proc. Natl. Acad. Sci. U.S.A. 82(19):6455-6459 (1985).

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$ 295.00
$ 99.00
Cat# AP1335A
(40 western blots)
Availability: In Stock
Bulk Size
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