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CCDC120 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCDC120 Antibody (N-term) AP13363a
    All lanes : Anti-CCDC120 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - CCDC120 Antibody (N-term) AP13363a
    CCDC120 Antibody (N-term) (Cat. #AP13363a) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the CCDC120 antibody detected the CCDC120 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96HB5
Other Accession NP_001156793.1, NP_001156794.1, NP_296375.1, NP_001156795.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 67567 Da
Antigen Region 59-88 aa
Additional Information
Gene ID 90060
Other Names Coiled-coil domain-containing protein 120, CCDC120
Target/Specificity This CCDC120 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human CCDC120.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCDC120 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCDC120
Function Centriolar protein required for centriole subdistal appendage assembly and microtubule anchoring in interphase cells (PubMed:28422092). Together with CCDC68, cooperate with subdistal appendage components ODF2, NIN and CEP170 for hierarchical subdistal appendage assembly (PubMed:28422092). Recruits NIN and CEP170 to centrosomes (PubMed:28422092). Also required for neurite growth. Localizes CYTH2 to vesicles to allow its transport along neurites, and subsequent ARF6 activation and neurite growth.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm. Cell projection, neuron projection. Cell projection, growth cone. Endosome Note=Localizes to the subdistal appendages of mother centrioles and proximal ends of both centrioles in interphase cells (PubMed:28422092) Recruited to subdistal appendages by ODF2 (PubMed:28422092). In differentiating neuroblastoma cells, colocalizes with CYTH2 in both neurite shaft and growth cone areas (PubMed:25326380). Partially colocalizes with endosomes along neurites in differentiating neuroblastoma cells (PubMed:25326380).
Research Areas
Citations (0)
citation

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Background

This gene encodes a protein that contains a coiled-coil domain. Four alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

References

Xin, X., et al. Genome Res. 19(7):1262-1269(2009)
Tarpey, P.S., et al. Nat. Genet. 41(5):535-543(2009)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)

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$ 365.00
$ 140.00
Cat# AP13363a
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