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TXNL1 (TRP32/TrxL) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TXNL1 (TRP32/TrxL) Antibody (C-term) AP1336b
    The anti-TrxL Pab (Cat. #AP1336b) is used in Western blot to detect TrxL in HL-60 cell lysate.
  • IHC-P - TXNL1 (TRP32/TrxL) Antibody (C-term) AP1336b
    Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody Cat # AP1336b gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jiménez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide, Cat # BP1336b). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43396
Other Accession Q920J4, Q8CDN6
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32251 Da
Antigen Region 256-289 aa
Additional Information
Gene ID 9352
Other Names Thioredoxin-like protein 1, 32 kDa thioredoxin-related protein, TXNL1, TRP32, TXL, TXNL
Target/Specificity This TXNL1 (TRP32/TrxL) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-289 amino acids from the C-terminal region of human TXNL1 (TRP32/TrxL).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTXNL1 (TRP32/TrxL) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TXNL1
Synonyms TRP32, TXL, TXNL
Function Active thioredoxin with a redox potential of about -250 mV.
Cellular Location Cytoplasm. Nucleus. Note=At least 85% of the cellular TXNL1 is proteasome-associated
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Thioredoxins are a group of small redox active proteins possessing a conserved active site sequence. TrxL (Thioredoxin like protein 1) has 2 distinct domains: an N terminal domain, which is 43% identical to human TXN, and a C terminal domain, which shows no homology to other proteins in the sequence databases. The active site sequence, located within the N terminal domain, is that of a thioredoxin like protein; compared to the active site sequence of TXN, it has a single amino acid substitution. Unlike other thioredoxin like proteins, TrxL does not serve as a substrate for thioredoxin reductase in an insulin assay.


Miranda-Vizuete, A., et al., Biochem. Biophys. Res. Commun. 243: 284-288 (1998).

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$ 295.00
$ 99.00
Cat# AP1336b
(40 western blots)
Availability: In Stock
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