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TAF8 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q7Z7C8 |
| Other Accession | Q5ZMS1, A7MAZ4, NP_612639.2 |
| Reactivity | Human |
| Predicted | Bovine, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 34262 Da |
| Antigen Region | 151-178 aa |
| Gene ID | 129685 |
|---|---|
| Other Names | Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43 kDa, TBP-associated factor 8, Transcription initiation factor TFIID 43 kDa subunit, TAFII-43, TAFII43, hTAFII43, TAF8, TAFII43, TBN |
| Target/Specificity | This TAF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-178 amino acids from the Central region of human TAF8. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TAF8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TAF8 |
|---|---|
| Synonyms | TAFII43, TBN |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473). TAF8 is involved in forming the TFIID-B module, together with TAF5 (PubMed:33795473). Mediates both basal and activator-dependent transcription (PubMed:14580349). Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts (PubMed:14580349). Required for the integration of TAF10 in the TAF complex (PubMed:14580349). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity). |
| Cellular Location | Nucleus. Cytoplasm Note=Predominantly nuclear. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
References
Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Soutoglou, E., et al. Mol. Cell. Biol. 25(10):4092-4104(2005)
Guermah, M., et al. Mol. Cell 12(4):991-1001(2003)
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