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COPS2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - COPS2 Antibody (N-term) AP13429a
    Fluorescent image of Hela cells stained with COPS2 Antibody (N-term)(Cat#AP13429a). AP13429a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • IHC-P - COPS2 Antibody (N-term) AP13429a
    Immunohistochemical analysis of paraffin-embedded H. skin section using COPS2 Antibody (N-term)(Cat#AP13429a). AP13429a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - COPS2 Antibody (N-term) AP13429a
    Western blot analysis of lysates from Hela, K562, U-251 MG cell line, mouse brain tissue, mouse F9 cell line, rat brain tissue (from left to right), using COPS2 Antibody (N-term)(Cat. #AP13429a). AP13429a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • IHC-P - COPS2 Antibody (N-term) AP13429a
    COPS2 Antibody (N-term) (Cat. #AP13429a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COPS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P61201
Other Accession Q6IR75, P61203, P61202, Q6IQT4, NP_004227.1
Reactivity Human
Predicted Zebrafish, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51597 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 9318
Other Names COP9 signalosome complex subunit 2, SGN2, Signalosome subunit 2, Alien homolog, JAB1-containing signalosome subunit 2, Thyroid receptor-interacting protein 15, TR-interacting protein 15, TRIP-15, COPS2, CSN2, TRIP15
Target/Specificity This COPS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human COPS2.
Dilution IF~~1:25
IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOPS2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COPS2
Synonyms CSN2, TRIP15
Function Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN- dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
Cellular Location Cytoplasm. Nucleus
Research Areas
Citations (0)

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Background

Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.

References

Kob, R., et al. Cell Cycle 8(13):2041-2049(2009)
Leal, J.F., et al. Oncogene 27(14):1961-1970(2008)
Fegers, I., et al. J. Proteome Res. 6(11):4182-4188(2007)
Tenbaum, S.P., et al. Biochim. Biophys. Acta 1773(9):1447-1454(2007)
Papaioannou, M., et al. Nucl Recept Signal 5, E008 (2007) :

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$ 295.00
$ 99.00
Cat# AP13429a
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(40 western blots)
Availability: In Stock
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